The net energy gain in glycolysis is the difference between the free energy of ATP hydrolysis and the free energy of ATP synthesis. In other words, it is the amount of energy that is released when one mole of glucose is converted into two moles of pyruvate.
The standard free energy of ATP hydrolysis is -7.3 kcal/mol, while the standard free energy of ATP synthesis is -6.8 kcal/mol. Therefore, the net energy gain in glycolysis is -0.5 kcal/mol.
This may not seem like much, but it is actually a very significant amount of energy. In fact, it is enough to drive the synthesis of two molecules of ATP from ADP.
So, in summary, the net energy gain in glycolysis is the difference between the free energy of ATP hydrolysis and the free energy of ATP synthesis. It is -0.5 kcal/mol, which is enough to drive the synthesis of two molecules of ATP from ADP.
Other related questions:
Q: What is the net gain of ATP and NADH in glycolysis?
A: In glycolysis, the net gain of ATP is 2 molecules and the net gain of NADH is 2 molecules.
Q: Why is there a net gain in glycolysis?
A: There is a net gain in glycolysis because it results in the production of more ATP than it consumes.
Q: What is the net gain of energy produced?
A: The net gain of energy produced is the total energy produced minus the total energy used.